Table 2.

Preparation of PCR Cocktail for BRCA1-3’UTR Gene variant

AS-PCR for G allele	1x	AS-PCR for T allele	1x
PCR master mix	10ul	PCR master mix	10ul
Common Forward primer F	0.25 ul	Common Forward primer F	0.25 ul
Wild Reverse primer R1	0.25 ul	Mutant Reverse R2	0.25 ul
Nuclease free water	12.50 ul	Nuclease free water	12.50 ul
DNA (50ng/ul)	2ul	DNA (50ng/ul)	2ul
Total volume	25ul	Total volume	25ul