MTT Cell Proliferation Assay. The effects of DAM and NDAM on the proliferation of H400 OSCC cells after 24, 48 and 72h. (A) DAM-treated H400 cell lines and (B) NDAM-treated H400 cell lines. Untreated control H400 cells exhibited an increase in OD from 24 to 72h after incubation. In contrast, treated cells after 24, 48 and 72h, showed decrease in OD from IC25 to IC75. Besides, the optical density of treated cells was lower than control in all tested concentration of compounds. Data are presented as mean ± SEM from three independent experiments (n=4). Significant differences were compared between control and treated cells (* p < 0.05 and **p < 0.01).