Figure 3.

Effects of 5-FU or Forbesione, or Their Combination, on the Expression of Apoptosis- Related Proteins in Ham-1 Cells. Representative western blots of lysates of cells treated with different concentrations of forbesione and 5-FU, either alone or in combination, for 24 h. Ham-1 cells were treated with various concentrations of two drugs, singly or in combination, for 24 h. The total cell lysates were separated on SDS-PAGE and transferred to nitrocellulose membranes. The membranes were probed with a primary antibody specific to Bcl-2, Bax, activated caspase-9, pro-caspase-3 or activated caspase-3. Staining intensities of apoptosis regulatory proteins were quantified by densitometry and normalized to β-actin. Data are expressed as mean ± SD of three independent experiments. Statistical analyses were performed using Student’s t-test, *p < 0.05, **p < 0.01 indicate a significant difference.