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. 2018;19(2):343–350. doi: 10.22034/APJCP.2018.19.2.343

Table 1.

Details of the Primers Used for the Sequences Amplified by PCR, Along with the Target Band Size and Annealing Temperatures are Given in Table Below

Genes and Primer sequences Annealing Temp. Amplicon size
CYP2D6*4: F: 5’- GCCTTCGCCAACCACTCCG-3’ 58°C 355 bp
CYP2D6*4: R: 5’- AAATCCTGCTCTTCCGAGGC-3’
CYP2D6*10: F: 5’- TCAACACAGCAGGTTCA -3’ 58°C 362 bp
CYP2D6*10: R: 5’- CTGTGGTTTCACCCACC -3’
CYP3A5*3:F: 5’- ATGGAGAGTGGCATAGGAGATA-3’ 58°C 130 bp
CYP3A5*3:R: 5’- TGTGGTCCAAACAGGGAAGAAATA -3’
CYP2C19*2:F: 5’- CAGAGCTTGGCATATTGTATC- -3’ 58°C 312 bp
CYP2C19*2:R: 5’- GTAAACACACAAAACTAGTCAATG -3’