Table 1.
Genes | Primers sequence (5’→ 3’) | Denaturation | Annealing Extension Cycle (no.) |
---|---|---|---|
AKAP4 | TCCAGCTCAGAAGGCAACT | 95 °C--- 30 s | 59 °C---30 s 72°C---25 s 35 |
CGCCCTCTGTGTCTCCTAC | |||
SPAG9 | GCAGTAAACAGCGAAGTG | 95 °C---30 s | 64.2 °C---30 s 72 °C ---25 s 40 |
CTTTTGTAGCCGAATGAGT | |||
CTAG1B | CGACAACATACTGACTATCCG | 94 °C---30s | 58 °C---30 s 72 °C---45s 35 |
CCTCCAGCGACAAACAATC |