Figure 4.

Growth hormone (GH) affects neither bone marrow dendritic cell (BM-DC) antigen presentation nor T cell activation. (A) OT-II mouse CD4⁺ splenocytes were stained with CellTrace Violet and co-cultured with bone marrow-derived wild-type (wt) or GHTg dendritic cells (BM-DC), alone or with rhGH. Cells were harvested after 48 or 72 h and analyzed by flow cytometry. A representative experiment is shown of three performed. wt cells at t = 0 were used as control. CD4⁺ cells from similar co-cultures were analyzed by flow cytometry at 0, 6, 12, and 24 h for activation markers CD25 (B), CD69 (C), and CD62L (D). One representative experiment is shown of three performed.