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. 2018 May 25;9:1165. doi: 10.3389/fimmu.2018.01165

Figure 6.

Figure 6

Effect of therapeutic rhGH administration on arthritic mice. Two-month-old DBA/1J mice were immunized with chicken type II collagen. (A) On appearance of the first signs of collagen-induced arthritis (score 2 = treatment day 0), affected mice were separated into two groups; one received a daily rhGH injection (2 µg/ml, 200 µl) until day 9, and the other received PBS as control. Clinical scores are expressed as the increase (Δ) from the initial score at t = 0 (score 2). Data shown as mean ± SEM. Student’s t-test (n = 12); *p ≤ 0.05. (B) Mean number of affected paws in arthritic mice as in (A). Data shown as mean ± SEM. Student’s t-test (n = 12); *p ≤ 0.05. (C) Total anti-collagen type II IgM and IgG were measured in mouse sera by ELISA at the end of the experiment (day 9 post-treatment) of vehicle- (PBS, black line) or rhGH- (gray line) treated arthritic mice as in (A). The figure shows mean absorbance at 492 nm ± SEM (n = 12). (D) Expression of characteristic Th17, Th1, and Treg transcription factors and cytokines in peripheral lymph nodes of mice, normalized to β-actin and expressed as 2−ΔΔCt. Data shown as mean ± SD. Student’s t-test **p ≤ 0.01; ***p ≤ 0.001.