Table 1.

PCR primer sequences.

Gene name	Genebank accession number	Sequence position	Primers	Sequence
β-Actin	NM007393	694–831	for rev	5′-AGAGGGAAATCGTGCGTGAC-3′ 5′-CAATAGTGATGACCTGGCCGT-3′
Tbx21	NM009322	474–574	for rev	5′-CACTAAGCAAGGACGGCGAA-3′ 5′-CCACCAAGACCACATCCACA-3′
RORγt	NM011281	808–908	for rev	5′-GAAGCTGGGAGCTATGCAGG-3′ 5′-TGGCTACGATGCAGCAAGGAG-3′
Foxp3	AY357713	929–1029	for rev	5′-TGCTGATGGGAGGAGATGTCT-3′ 5′-TTTCTTTCAGGGACAGCCTGTT-3′
IFNγ	K00083	929–1029	for rev	5′-TGCTGATGGGAGGAGATGTCT-3′ 5’-TTTCTTTCAGGGACAGCCTGTT-3′
IL-2	NM008366	252–352	for rev	5′-GAAACTCCCCAGGATGCTC A-3′ 5′-GCCGCAGAGGTCCAAGTTC-3′
IL-10	NM010548	113–218	for rev	5′-TGACTGGCATGAGGATCAGC-3′ 5′-AGTCCGCAGCTCTAGGAGCA-3′
IL-17A	NM010552	338–398	for rev	5′-GAAGCTCAGTGCCGCCA-3′ 5′TTCATGTGGTGGTCCAGCTTT-3′
IL-21	NM021782	510–581	for rev	5′-AAGATTCCTGAGGATCCGAGAA-3′ 5′-GCATTCGTGAGCGTCTATAGTGTC-3′
IL-22	NM016971	204–271	for rev	5′-TACATCGTCAACGCACCTTT-3′ 5′-CGGACGTCTGTGTTGTTAT-3′
IL-6	NM031168	187–327	for rev	5′-GAGGATACCACTCCCAACAGACC-3′ 5′-AAGTGCATCATCGTTGTTCATACA-3′
TGFβ1	NM011577	1697–1790	for rev	5′-GGAGAGCCCTGGATACCAAC-3′ 5′-CAACCCAGGTCCTTCCTAAA-3′
CCL21a	NM001193667	250–378	for rev	5′-ATCCCGGCAATCCTGTTCTC-3′ 5′-GGGGCTTTGTTTCCCTGGG-3′
CCL20	NM001159738	107–224	for rev	5′-CTGCTGGCTCACCTCTGCA-3′ 5′-CATCGGCCATCTGTCTTGTG-3′
CXCL12	NM021704	244–344	for rev	5′-AGCCAACGTCAAGCATCTGA-3′ 5′-TCGGGTCAATGCACACTTGT-3′

Primer sequences employed for PCR detection of several factors involved in the differentiation and function of Th17, Th1, or Treg subsets, as well as for β-actin (housekeeping gene).