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. 2018 May 25;9:1086. doi: 10.3389/fmicb.2018.01086

FIGURE 1.

FIGURE 1

Detection of hVISA features. (A) Population analysis profile of vancomycin on CGK5 and its mutant derivatives. Overnight cultures of test isolates were serially diluted in Trypticase Soy broth (TSB) and inoculated onto brain heart infusion (BHI) agar plates containing 0 to 8 μg/ml of vancomycin. After 48 h incubation at 35°C, colonies were counted and the results were plotted on a graph. Mu3 (ATCC 700698) and N315 S. aureus strains were tested in parallel as hVISA positive and negative controls, respectively. (B) Transmission electron microscopy images of CGK5 and its mutant derivatives. Cell wall thickness (in nanometers) was measured at 150,000× magnification. Fifty measurements of equatorially cut cells were taken for calculation of cell wall thickness, and results are expressed as mean ± SD. CGK5, progenitor of CGK5mut; CGK5mut, derivatives of CGK5 with L431F mutation in mprF; CGK5mutR, reverse complementation construct of CGK5mut.