FIGURE 2.

Growth curve of CGK5, CGK5mut, and CGK5mutR in Mueller Hinton II broth (MHB). Overnight fresh cultures of the bacteria were adjusted in 0.85% NaCl to 0.5 McFarland turbidity, then diluted 1:200 in MHB to obtain ∼5 × 10⁵ CFU/ml starting inoculum. The inoculum was dispensed at 120 μl per well in triplicates to a 100-well plate and incubated at 37°C in Bioscreen C MBR. The OD₆₀₀ of each well was read every 30 min for 24 h. The average OD of the blank wells was subtracted from the average of the triplicate test wells at each time point and plotted.