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. 2018 May 25;9:1089. doi: 10.3389/fmicb.2018.01089

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Copyright © 2018 Lin, Xu, Yan, Zhang, Li, Chen, Chen and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of differentially expressed genes identified by mRNA-Seq. HD11 cells (A) or DF-1 cells (B) were infected with ALV-J or medium as a control. Total RNA was isolated and subjected to qRT-PCR to validate the differentially expressed genes identified by mRNA-Seq. Expression levels of target genes were calculated relative to the expression of the GAPDH gene and expressed as fold increase or decrease relative to the control samples. Results are representative of three independent experiments. Data are represented as means ± SD. ^∗∗P < 0.01; ^∗P < 0.05.