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. 2018 May 25;9:1087. doi: 10.3389/fmicb.2018.01087

FIGURE 2.

FIGURE 2

GC-FID chromatograms of diesel oil extracted at 7th day from (A) control and (B) experimental flask in biodegradation study with endophytic isolate L10. Degradation of diesel oil was carried out under aerobic conditions at 30°C in 300-mL Erlenmeyer flasks containing 100 mL of MS medium and 5 g/L of diesel oil. Control was performed by inoculating with the boiled cells of isolate. Hydrocarbons were extracted from the culture medium with an equal volume of n-hexane, analyzed using a gas chromatograph equipped with an FID detector (GC-FID, 7890A, Agilent, United States), and quantified in comparison to the n-alkane homologous series.