Figure 1. Crystal structure of M2PYK.

M2PYK is a homotetramer that consists of an A-domain (green, residues 44–116 and 219–402); B-domain (cyan, residues 117–218); C-domain (yellow, residues 403–531); and N-terminal domain (blue, residues 13–43. Residues 1–12, as well as additional residues from the His₆-tagged construct that were too disordered to be solved and shown in the structures). The only unconserved region between M1PYK and M2PYK is highlighted in red. Positions of the active site, the amino-acid binding pocket, and the FBP-binding pocket are also highlighted. The large (A–A) and small (C–C) interfaces are shown as dashed lines.