Table 3.
Effect of normalisation method on the correlation of CVF cytokine concentration and specimen weight.
| Cytokine | Normalisation method | Median (IQR) | Spearmans (ρ) | P value |
|---|---|---|---|---|
| Pro-inflammatory | ||||
| IL-1β | As measured (pg/mL) | 58 (20–443) | −0.401 | 0.004 |
| Dilution (pg/mL) | 116 (44–1441) | −0.365 | 0.008 | |
| Protein (pg/mg) | 2 (1–14) | −0.351 | 0.013 | |
| IL-6 | As measured (pg/mL) | 8 (3–40) | −0.348 | 0.012 |
| Dilution (pg/mL) | 24 (8–119) | −0.325 | 0.02 | |
| Protein (pg/mg) | 0.3 (0.1–1.6) | −0.359 | 0.01 | |
| IL-8 | As measured (pg/mL) | 621 (141–3170) | −0.281 | 0.046 |
| Dilution (pg/mL) | 2636 (281–17,248) | −0.252 | 0.075 | |
| Protein (pg/mg) | 202 (36–453) | −0.264 | 0.067 | |
| TNF-α | As measured (pg/mL) | 0.6 (0.2–1.6) | −0.166 | 0.243 |
| Dilution (pg/mL) | 2 (0–5) | −0.168 | 0.238 | |
| Protein (pg/mg) | 0.02 (0.006–0.07) | −0.179 | 0.212 | |
| Immuno-regulatory | ||||
| IFN-γ | As measured (pg/mL) | 3 (1–6) | −0.293 | 0.037 |
| Dilution (pg/mL) | 6 (3−20) | −0.241 | 0.089 | |
| Protein (pg/mg) | 0.1 (0.04–0.3) | −0.217 | 0.130 | |
| IL-2 | As measured (pg/mL) | 0.5 (0.3–0.9) | −0.258 | 0.068 |
| Dilution (pg/mL) | 1 (0.6–3.7) | −0.198 | 0.164 | |
| Protein (pg/mg) | 0.02 (0.006–0.04) | −0.252 | 0.077 | |
| IL-4 | As measured (pg/mL) | 0.1 (0.06–0.4) | −0.272 | 0.053 |
| Dilution (pg/mL) | 0.4 (0.1–1.1) | −0.250 | 0.077 | |
| Protein (pg/mg) | 0.005 (0.002–0.11) | −0.208 | 0.147 | |
| IL-10 | As measured (pg/mL) | 0.7 (0.3–1.7) | −0.119 | 0.407 |
| Dilution (pg/mL) | 3 (1–6) | −0.098 | 0.496 | |
| Protein (pg/mg) | 0.02 (0.01–0.09) | −0.119 | 0.411 | |
| IL-12 | As measured (pg/mL) | 0.2 (0.1–0.7) | −0.048 | 0.739 |
| Dilution (pg/mL) | 0.6 (0.2–3.3) | −0.016 | 0.909 | |
| Protein (pg/mg) | 0.007 (0.003–0.05) | −0.042 | 0.770 | |
| IL-13 | As measured (pg/mL) | 4 (2–7) | −0.210 | 0.136 |
| Dilution (pg/mL) | 12 (5–35) | −0.137 | 0.336 | |
| Protein (pg/mg) | 0.1 (0.05–0.4) | −0.193 | 0.180 | |