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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Liver Transpl. 2016 Aug;22(8):1115–1128. doi: 10.1002/lt.24473

FIG. 5.

FIG. 5

IHC for neutrophilic infiltration. To evaluate the extent of neutrophil infiltration in the different groups, IHC with a Ly6-G antibody was performed. Ly6-G is a protein expressed on neutrophils, and thus serves as a marker of neutrophil infiltration. Representative slides for (A) LysMcre+/caNrf2− sham (n = 4), (B) LysMcre+/caNrf2− IR (n = 7), (C) LysMcre+/caNrf2+ sham (n = 4), and (D) LysMcre+/caNrf2+ IR (n = 5) animals are shown. There is minimal neutrophilic infiltrate in the sham groups of both genotypes and the LysMcre+/caNrf2+ IR group. However, there is a marked increase in neutrophilic infiltrate in the LysMcre+/caNrf2− IR group. (E) The number of Ly6-G positive cells was quantified for each experimental group. Confirmatory to our visual findings, we see significantly increased numbers of Ly6-G staining cells in the LysMcre+/caNrf2− IR group as compared to its corresponding sham group. We also see significantly decreased numbers of Ly6-G positive cells in the LysMcre+/caNrf2+ IR group as compared to the LysMcre+/caNrf2− IR group. * P < 0.05 as compared to the sham group of the same genotype. # P < 0.05 as compared to +/− group of the same treatment.