FIG. 7.

Inflammation and oxidative stress after partial hepatic IRI. (A) RNA was isolated from livers of experimental animals, and quantitative RT-PCR of some classical proinflammatory cytokines was performed. There were significant increases in mRNA abundance of IL6, TNF-α, and CCL2 in the LysMcre+/caNrf2− IR animals, as compared to its sham group. When comparing the LysMcre+/caNrf2+ IR group to the LysMcre+/caNrf2− IR group, there were significant decreases in IL1β, IL6, TNF-α, CCL2, and CXCL10. (B) To assess the degree of oxidative stress, 8-isoprostane levels in the livers were assessed. Hepatic 8-isoprostane levels were significantly elevated in IR groups of both genotypes as compared to their sham counterparts. Hepatic 8-isoprostane levels were significantly decreased in the LysMcre+/caNrf2+ IR group as compared to the LysMcre+/caNrf2− IR group. * P < 0.05 as compared to the sham group of the same genotype. # P < 0.05 as compared to +/− group of the same treatment.