TABLE 5.
MS analysis and yields of leaderless peptides used for activity testa
| Name | Predicted mass (Da) (no. of dehydrations measured/no. of possible dehydrations) | Measured mass (Da) | Inferred degradation | Yield (μg/liter) |
|---|---|---|---|---|
| T6F | 4,160.07 (7/9) | 4,158.66 | No | 834 |
| T6S | 3,652.44 (7/8) | 3,654.34 | No | 940 |
| T6m1 | 3,780.61 (7/8) | 3,778.06 | No | 1,218 |
| T6m2 | 3,822.69 (7/8) | 3,822.82 | No | 1,138 |
| T6m3 | 3,676.52 (8/8) | 3,678.67 | No | 740 |
| T6m4 | 3,768.60 (8/9) | 3,766.75 | No | 1,460 |
| T8F | 4,186.14 (9/9) | 4,186.03 | ΔAL | 740 |
| T8S | 3,324.08 (6/8) | 3,322.60 | ΔVALKAL | 640 |
| T8m1 | 3,696.83 (8/8) | 3,695.53 | ΔALK | 246 |
| T8m2 | 3,777.70 (6/8) | 3,777.98 | ΔALK | 299 |
| T8m3 | 3,366.16 (6/8) | 3,366.81 | ΔVALKAL | 260 |
| T8m4 | 4,035.99 (7/9) | 4,033.09 | No | 414 |
| GNT16 | 4,324.32 (7/9) | 4,326.29 | No | 340 |
| GNT16SG | 3,816.69 (7/8) | 3,815.64 | No | 264 |
| T16m1 | 3,944.87 (7/8) | 3,941.44 | No | 234 |
| T16m2 | 3,966.00 (8/8) | 3,966.68 | No | 498 |
| T16m3 | 3,855.90 (7/8) | 3,854.75 | No | 316 |
| T16m4 | 3,932.85 (8/9) | 3,930.71 | No | 483 |
| Nisin | 3,352.61 (8/9) | 3,354.75 | No | NDb |
a
Nisin was purified from a commercial 2.5% preparation.
b
ND, not determined.