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Characterization of cezomycin-CoA CalG thioesterase hydrolytic activity. (A) SDS-PAGE analysis of purified CalG protein. (B) HPLC analysis of the CalG hydrolysis reaction products. (C) High-resolution mass spectrometry analysis of cezomycin-CoA and CalG reaction product as well as CoA and cezomycin. The mass fragment peak at m/z of 94 is characteristic of the pyrrole moiety, and that at m/z of 160 is characteristic of benzoxazole. Mass fragments of CoA are marked.
