TABLE 3.
Primers used in this study
| Primer | Sequence (5′–3′)a | Use |
|---|---|---|
| G-F1 | CAGTACCCGGGGCGCCAGGACCGGCACCACGAGCCCATGATTCCGGGGATCCGTCGACC | Replacement of calG by Redirect Technology |
| G-F2 | ATGGCTCGCCTTGGGGTCGTCGTCTCCGGTCAGCGCGGTTGTAGGCTGGAGCTGCTTC | |
| G-F3 | ATGACCGCCACCGATCCGTG | PCR analysis of GLX16 (ΔcalG) |
| G-F4 | TCAGCGTGGCGCGACGGCGG | |
| G-F5 | CCGGAATTCTTGTGCGACCCGGCCGACTA | PCR amplification of calG for complementation |
| G-F6 | GGAATTCCATATGACCGCCACCGATCCGTG | |
| 28aG-F7 | GAATTCCATATGACCGCCACCGATCCGTGGATACG | Amplification of calG for expression |
| 28aG-F8 | CTTCCTCGAGTCAGCGTGGCGCGACGGCGGACTC |
a
Boldface indicates the 20-nt (G-F1) and 19-nt (G-F2) sequences for amplification of the apramycin resistance gene.