FIG 2.

S. oneidensis may not generate NO endogenously. (A and B) Growth and NO production of wild-type (WT) (A) and ΔnrfA (B) strains in LB under aerobic conditions. Growth and samples were measured and collected at indicated time points, and nitrite concentrations in supernatants were determined. Dashed and solid lines represent growth and nitrite concentrations, respectively. Samples of cells without and with the Bacillus subtilis nosA gene are in blue and red, respectively. (C) Representative NO production of S. oneidensis and S. denitrificans under anaerobic conditions. Cultures of all strains grown on TMAO to an OD₆₀₀ of ∼0.3 were collected, washed, and suspended in the same medium containing 5 mM nitrite. Data for NO production from both strains in the presence of NO scavenger carboxy-PTIO at 0.1 mM were included. The dissolved NO concentrations were monitored using an NO-specific electrode. Shown are either representative data or means ± SEMs from at least three experiments.