TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| E. coli DH5α | Host for cloning | Lab stock |
| E. coli MG1655 | Wild type | Coli Genetic Stock Center |
| E. coli WM3064 | Donor strain for conjugation, ΔdapA | W. Metcalf, UIUC |
| B. subtilis 168 | Source of bacterial bNOS | Bacillus Genetic Stock Center |
| S. denitrificans | Shewanella denitrifying strain OS217 | DSM 15013 |
| S. oneidensis | ||
| MR-1 | Wild type | ATCC 700550 |
| HG0039 | ΔSO_0039 derived from MR-1 | This study |
| HG0504 | Δfre derived from MR-1 | This study |
| HG1363 | Δhcp derived from MR-1 | This study |
| HGCCO | Δcco (ΔccoNOPQ) derived from MR-1 | 50 |
| HG2805 | ΔnnrS derived from MR-1 | This study |
| HG3980 | ΔnrfA derived from MR-1 | 38 |
| HGCYD | Δcyd (ΔcydABX) derived from MR-1 | 66 |
| Plasmids | ||
| pHGM01 | Apr, Gmr, Cmr, att-based suicide vector | 27 |
| pHG102 | Kmr, ParcA expression vector | 63 |
| pHGEI01 | Integrative E. coli lacZ reporter vector | 41 |
| pBBR-Cre | Helper vector for antibiotic marker removal | 29 |
| pHGE-Ptac | Kmr, IPTG-inducible Ptac expression vector | 48 |
| pHGE-Ptac-cco | Inducible expression of cco | 50 |
| pHGE-Ptac-cyd | Inducible expression of cyd | 50 |
| pHGE-Ptac-nrfA | Inducible expression of nrfA | 30 |
| pHGE-Ptac-acnB | Inducible expression of acnB | This study |
| pHGE-Ptac-ilvD | Inducible expression of ilvD | This study |
| pHGE-Ptac-lpdA | Inducible expression of lpdA | This study |
| pHG102-nosA | Forced expression of nosA | This study |
| pHGEI-PnrfA-lacZ | E. coli lacZ under the control of nrfA promoter | 30 |
| pHGEI-PSO0039-lacZ | E. coli lacZ under the control of SO_0039 promoter | This study |
| pHGEI-Phcp-lacZ | E. coli lacZ under the control of hcp promoter | This study |
| pHGEI-PnnrS-lacZ | E. coli lacZ under the control of nnrS promoter | This study |
| pHGEI-Pfre-lacZ | E. coli lacZ under the control of fre promoter | This study |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Gmr, gentamicin resistance; Kmr, kanamycin resistance.