TABLE 3.
Plasmids and primers used for GFP labeling of S. aureus strain LUG2902
Plasmid or primer | Description or sequencea | Source |
---|---|---|
Plasmids | ||
pACL1484 | Promoterless gfpuvr shuttle vector | A. Cheung |
pLUG1283 | 16S rRNA gene promoter cloned into EcoRI-XbaI site of pACL1484 | This study |
Primers | ||
P16s-for-EcoRI | CGGAATTCAGCGTAAGGAATTACACTTT | This study |
P16s-rev-XbaI | GCTCTAGATGATGTTTGATTAGCTCATAAATAC | This study |
Restriction sites are underlined.