TABLE 2.

Virucidal efficacy of silver dihydrogen citrate against FCV on glass measured by plaque assay and qRT-PCR

Contact time (min)	Reductiona
	Plaque assay (log10 PFU)		qRT-PCR (log10 copies)
	With 5% serumb	Control without 5% serumc	With 5% serumb	Control without 5% serumc
1	1.17 ± 0.30 AA	0.77 ± 0.41 AA	0.12 ± 0.25 AA	−0.85 ± 0.09 AB
5	3.71 ± 0.35 BA	2.49 ± 0.08 BB	0.53 ± 0.07 ABA	−0.58 ± 0.26 AB
10	3.84 ± 0.45 B	>3.46 A	0.45 ± 0.06 BA	−0.66 ± 0.10 AB
30	>4.66 C	>3.46 A	1.01 ± 0.28 CA	−0.45 ± 0.38 AB

^aThe data are expressed as means ± SDs from 9 replicates in 3 independent experiments. Values with different nonsuperscript letters in the same column are significantly different (P < 0.05), whereas values with different superscript letters in the same row for each detection method are significantly different.

^bFCV stocks concentrated via ultrafiltration were diluted in complete Dulbecco's modified Eagle's medium with 5% low-endotoxin (<10 EU/ml) fetal bovine serum. Recovered control was 5.68 log₁₀ ± 0.24 log₁₀ PFU.

^cFCV stocks concentrated via ultrafiltration were diluted in 1× phosphate-buffered saline. Recovered control was 4.50 log₁₀ ± 0.04 log₁₀ PFU.