TABLE 3.
Virucidal efficacy of steam vapor with TANCS against FCV on glass measured by plaque assay and qRT-PCR
| Contact time (s) | Reductiona |
|||
|---|---|---|---|---|
| Plaque assay (log10 PFU) |
qRT-PCR (log10 copies) |
|||
| With 5% serumb | Control without 5% serumc | With 5% serumb | Control without 5% serumc | |
| 10 | >4.93 A | >4.11 A | 2.31 ± 0.25 AA | 0.70 ± 0.32 AB |
| 30 | >4.93 A | >4.11 A | 1.92 ± 0.21 AA | 0.20 ± 0.10 AB |
| 60 | >4.93 A | >4.11 A | 1.94 ± 0.07 AA | 0.30 ± 0.38 ABB |
| 90 | >4.93 A | >4.11 A | 1.93 ± 0.32 AA | 1.03 ± 0.36 BB |
The data are expressed as means ± SDs from 9 replicates in 3 independent experiments. Values with different nonsuperscript letters in the same column are significantly different (P < 0.05), whereas values with different superscript letters in the same row for each detection method are significantly different.
FCV stocks concentrated via ultrafiltration were diluted in complete Dulbecco's modified Eagle's medium with 5% low-endotoxin (<10 EU/ml) fetal bovine serum. Recovered control was 5.99 log10 ± 0.20 log10 PFU.
FCV stocks concentrated via ultrafiltration were diluted in 1× phosphate-buffered saline. Recovered control was 5.17 log10 ± 0.16 log10 PFU.