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. 2018 May 25;9:1092. doi: 10.3389/fmicb.2018.01092

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Copyright © 2018 Martínez-Gómez, Ståhle, Gil-Ramírez, Zúñiga-Ripa, Zaccheus, Moriyón, Iriarte, Widmalm and Conde-Álvarez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Brucella abortus wbdR constructs display increased sensitivity to normal serum but not to bactericidal polycations. Polymyxin B sensitivity (representative results of three independent experiments) (A), surface charge (Z potential) (mean of ± SD of three independent experiments) (B) and survival after 90 min of incubation in normal bovine serum (media ± standard error of three technical replicates representative of three independent experiments) (C) of Ba-parental, Ba::Tn7wbdR, Ba::Tn7wbdRΔwbkC and the R mutant BaΔper.