Figure 5.

Attenuated interferon-regulated gene expression in the bone marrow and peripheral organs of LPS-challenged mice. Semiquantitative measurement of the indicated mRNAs relative to 18S rRNA by RT-PCR in (A) total lung, (B) kidney tissue, and (C) FACS-enriched bone marrow resident Gr1CD11b^POS cells of wild-type mice, mice lacking PAR2 or EPCR, and from TF^LOW mice with reduced hematopoietic cell TF expression. Data are expressed as the fold increase relative to LPS-challenged wild-type mice and represent the average ± standard deviation from triplicate measurements of a single pooled sample generated by combining equal amounts of RNA prepared from 4 individual mice before reverse transcription (for lung and kidney tissue) or from 1 sample of RNA prepared from Gr1CD11b^POS cells isolated via FACS from the pooled bone marrows of 5 mice each. *P < .05 compared with wild-type controls.