Table 1. PCR detection of eDNA samples from containers with different volumes of water, numbers of larvae and occupation times.

No. of larvae	Vol. of water	Larvae density (larvae/mL)	Hours	PCR results	Band strength
1	50 mL	0.02	1	0/3	No band
3	50 mL	0.06	1	3/3	Weak
6	50 mL	0.12	1	3/3	Weak
0	50 mL	0	1	0/1	No band
1	50 mL	0.02	6	1/3	Weak
3	50 mL	0.06	6	3/3	Weak
6	50 mL	0.12	6	2/3	Weak
0	50 mL	0	6	0/1	No band
1	50 mL	0.02	24	2/3	Weak
3	50 mL	0.06	24	3/3	Weak
6	50 mL	0.12	24	3/3	Mid
0	50 mL	0	24	0/1	No band
2	1 L	0.002	24	3/3	Weak
5	1 L	0.005	24	3/3	Weak
10	1 L	0.01	24	3/3	Mid
20	1 L	0.02	24	3/3	Strong
0	1 L	0	24	0/1	No band