Table 2. Comparison of results from IGS and cyt b TaqMan assays showing the number of positive samples in each of the four replicates and the average concentrations (pg) for each sample.

The larval density was a fivefold dilution series (d1,2,3) for the initial number of larval in water sample, C. Dilutions whose eDNA concentration was not determined are noted as n.d. Minimum sample dilutions at which eDNA was detected are highlighted in bold.

2 larvae						5 larvae
		Density	Positives	Av Conc	Std Dev			Density	Positives	Av Conc	Std Dev
IGS	C	2	2/4	0.0002	0.0002	IGS	C	5	4/4	0.006	0.002
	d1	0.4	0/4	n.d	n.d		d1	1	3/4	0.003	0.002
	d2	0.08	0/4	n.d	n.d		d2	0.2	0/4	n.d	n.d
	d3	0.016	0/4	n.d	n.d		d3	0.04	2/4	0.003	0.008
	NTC	0	0/2	n.d	n.d		NTC	0	0/2	n.d	n.d
cyt b	C	2	2/4	0.0306	0.0422	cyt b	C	5	4/4	0.0885	0.0244
	d1	0.4	0/4	n.d	n.d		d1	1	1/4	0.05101	0
	d2	0.08	0/4	n.d	n.d		d2	0.2	0/4	n.d	n.d
	d3	0.016	0/4	n.d	n.d		d3	0.04	0/4	n.d	n.d
	NTC	0	0/2	0	0		NTC	0	0/2	0	0
10 larvae						20 larvae
		Density	Positives	Av Conc	Std Dev			Density	Positives	Av Conc	Std Dev
IGS	C	10	4/4	0.0078	0.0035	IGS	C	20	4/4	9.0527	1.0964
	d1	2	4/4	0.0017	0.0010		d1	4	4/4	1.7046	0.0821
	d2	0.4	2/4	0.0004	0.0002		d2	0.8	4/4	0.3758	0.0884
	d3	0.08	1/4	0.0002	0		d3	0.16	4/4	0.0818	0.0182
	NTC	0	0/2	0	0		NTC	0	0/2	0	0
cyt b	C	10	4/4	0.7848	0.4672	cyt b	C	20	4/4	27.2678	5.5316
	d1	2	3/4	0.2078	0.1813		d1	4	4/4	6.8140	1.8393
	d2	0.4	1/4	0.1270	0*		d2	0.8	4/4	1.7338	0.2231
	d3	0.08	1/4	0.0346	0		d3	0.16	4/4	0.3709	0.1233
	NTC	0	0/2	0	0		NTC	0	0/2	0	0