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. 2018 May 31;8:8460. doi: 10.1038/s41598-018-26828-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TTS suppressed inflammatory cytokines release and renal injury makers in BALB/C mice. Mice were pretreated with TTS (40 and 80 mg/kg, i.p.), TTC (80 mg/kg) for 2 h before LPS (10 mg/kg, i.p.) injection. After 12 h, the blood samples were collected via retro-orbital route under anesthesia. The inflammatory cytokines like TNF-α (A), IL-6 (B) and IL-1β (C) and creatinine (D) and blood urea nitrogen (E) in serum were determined by ELISA kits. DEX (5 mg/kg, i.v.) was used as positive control. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, compared to LPS alone group (n = 10).