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. 2018 May 31;9(6):664. doi: 10.1038/s41419-018-0675-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a qPCR and western blot analyses of STX2 in specific shRNA-transduced stable cells derived from SW620 and LOVO. b, c The migratory and invasive properties of the stable cell lines were analyzed using Boyden chambers or Matrigel-coated Boyden chambers. Error bars represent the mean ± s.d. from three independent experiments. d, e Wound-healing assay. Histograms represent the average migrated distances at the indicated times. Error bars represent the mean ± s.d. from three independent experiments. f Three-dimensional morphology analysis. Histograms represent the average number of filopodia formed by each cell sphere from three independent experiments. Error bars represent the mean ± s.d. g Representative gross and microscopic images of the intestines and livers are shown. The sections were stained with H&E. The arrows indicate the primary tumors in the intestines, visible metastatic nodules in the liver and infiltrative tumors in the intestine. h The number of visible metastatic nodules in the liver. i Overall survival times of the different group of mice bearing liver metastases. **p < 0.01