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. 2018 May 31;8:8438. doi: 10.1038/s41598-018-26860-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DP1 knockdown does not reduce E2F1 activation of the ARF promoter or binding of deregulated E2F1. (A) Reporter analysis of CDC6 and ARF promoters by ectopic expression of E2F1 in WI-38 cells expressing DP1-shRNA. *P < 0.05. (B) Gel mobility shift assays with extracts from WI-38 cells ectopically expressing E2F1 and DP1-shRNA. Probes were a DHFR promoter fragment containing typical E2F sites (left panel) and EREA (right panel). Competitors were an adenovirus E2 enhancer fragment containing typical E2F sites (E2 wt) and its mutant (E2 mt). Ad-shCon and Ad-shDP1-2 data were on the same gels with the same exposure (Supplementary Fig. S7). (C) ChIP analysis of E2F target promoters in WI-38 cells ectopically expressing E2F1 and DP1-shRNA. Full-length gels are presented in Supplementary Fig. S8.