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. 2018 May 31;8:8438. doi: 10.1038/s41598-018-26860-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DP family proteins do not contribute to deregulated E2F-induced ARF mRNA expression or activation of p53. (A,B) RT-PCR analysis of mRNA induction by ectopic expression of E2F1 (A) or E1a (B) in WI-38 cells expressing DP1-shRNA. Full-length gels are presented in Supplementary Figs S15(A) and S16(B). (C,D) Immunoblot analysis of DP1 expression and induction of ARF and p53 by ectopic expression of E2F1 (C) or E1a (D) in WI-38 cells expressing DP1-shRNA. Full-length blots are presented in Supplementary Figs S17(C) and S18(D). (E–G) Reporter analyses in WI-38 cells ectopically expressing E2F1 and DP2 (E) or DP3 (F,G). *P < 0.05, **P < 0.01. (H) Immunoblot analysis of DP family proteins in 293 cells transfected with expression plasmid for respective DP family proteins. Full-length blots are presented in Supplementary Fig. S19.