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. 2018 May 31;8:8438. doi: 10.1038/s41598-018-26860-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DP1 does not affect E2F-induced apoptosis. (A) DP1 knockdown suppresses DNA synthesis induced by serum stimulation or expression of E1a. FACS analysis of BrdU-positive cells in WI-38 cells expressing DP1-shRNA stimulated with serum or expressing E1a. **P < 0.01. (B) DP1 knockdown suppresses cell proliferation induced by serum stimulation. Growth curve of WI-38 cells expressing DP1-shRNA. **P < 0.01. (C–F) DP knockdown does not reduce deregulated E2F-induced apoptosis. (C,D) Percentage of cells with a sub-G₁ DNA content (C) and annexin V-stained cells (D) in WI-38 cells infected with Ad-shDP1s and either Ad-E2F1 or Ad-12SE1A. **P < 0.01. (E,F) Percentage of cells with a sub-G₁ DNA content (E) and annexin V-stained cells (F) in WI-38 cells infected with Ad-shDP1 and/or Ad-shDP2, and either Ad-E2F1 or Ad-12SE1A. **P < 0.01. (G,H) DP1 does not contribute to E2F1-induced apoptosis in WI-38 cells. Percentage of sub-G₁ population (left panel) and annexin V-positive cells (right panel) in WI-38 cells expressing E2F1 and/or DP1. WI-38 cells were infected with Ad-E2F1 at low MOI (20) (G) or high MOI (150) (H). **P < 0.01.