Table 1.

Establishing the frequency of HCMV-specific T- and B-memory cells in seronegative human donors. For testing T cells, the PBMC of the donors specified by serial numbers were plated at 3 × 10⁵ cells per well, with HCMVpp65 peptide pool at μg/mL, or I-HCMV at 50 μg/mL, as specified. Each condition was tested in triplicate wells, and the mean IFN-γ SFU numbers are shown. The medium control for all donors was less than 10 SFU per well (not shown). Statistical analysis was performed comparing the SFU counts in the three medium control wells with the three replicate wells for HCMVpp65 or I-HCMV. Test results that reached statistical significance (as defined in Materials and Methods) are highlighted in green. Test results exceeding 150 SFU/well, and therefore above the detection limit of flow cytometry, are highlighted in dark green. For B-cell testing, plates were coated with 50 μg/mL I-HCMV and 5 × 10⁵ preactivated PBMC were plated per well. The mean pan-IgG SFU numbers of three replicate wells are specified for each donor. The Donor Serial IDs were assigned after sorting them into the four response categories specified in Figure 4.

Donor Serial #	T-cells		B-cells	Donor Serial #	T-cells		B-cells
	HCMVpp65	I-HCMV	I-HCMV		HCMVpp65	I-HCMV	I-HCMV
1	122	362	45	42	133	515	not perf.
				43	353	661	not perf.
2	668	111	45	44	847	721	not perf.
3	189	77	101	45	281	890	not perf.
4	403	52	21	46	31	20	25
5	29	465	20	47	not perf.	>1000	26
6	243	440	85	48	196	48	9
7	345	459	59	49	2	23	21
8	515	>1000	76	50	27	16	152
9	280	237	38	51	2	73	11
10	607	646	62	52	86	17	17
11	385	764	33	53	21	82	1
12	357	708	13	54	5	104	2
13	401	480	13	55	3	169	1
14	835	>1000	133	56	1	71	1
15	192	435	29	57	5	148	1
16	72	522	23	58	1	74	3
17	551	382	95	59	1	413	2
18	162	385	72	60.	0	83	not perf.
19	792	697	43	61	5	79	not perf.
20	237	336	133	62	3	19	2
21	506	611	89	63	0	46	not perf.
22	533	473	128	64	1	49	not perf.
23	567	433	78	65	0	40	1
24	515	422	48	66	0	26	2
25	23	79	104	67	0	46	1
26	266	193	110	68	1	23	1
27	95	194	72	69	2	4	2
28	465	153	79	70	2	3	1
29	145	202	8	71	2	4	0
30	65	239	8	72	2	4	2
31	298	461	6	73	2	8	3
32	271	519	3	74	2	8	3
33	163	50	4	75	3	1	1
34	403	234	0	76	3	1	6
35	68	148	2	77	5	4	not perf.
36	301	420	2	78	5	4	not perf.
37	176	87	1	79	3	9	not perf.
38	88	433	4	80	1	8	not perf.
39	90	110	5	81	1	3	not perf.
40	52	766	3	82	0	0	not perf.
41	69	239	not perf.	83	1	1	not perf.