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. 2018 May 31;8:8457. doi: 10.1038/s41598-018-26903-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation between chicken ACSF2 mRNA expression and laying performance. (a–c) Expression pattern of ACSFs in livers from CEP and IEP. (d–f) Expression pattern of ACSFs in ovaries from CEP and IEP. (g) Expression pattern of ACSF2 in ovaries from different developmental stages. (h) The changing trends of ACSF2 expression and laying rate per week in Lushi green-shelled-egg chickens. The housekeeping gene β-actin was used as an internal standard to estimate mRNA relative expression. All data were shown as fold change compared with gene expression in CEP. Data were represented as Mean ± SD (n = 8). *Significant differences (P < 0.05), **highly significant differences (P < 0.01).