Figure 2.

Effects of Myricetin-derivatives on E. coli biofilm formation. (a) Biofilms of E. coli BW25113 were formed in YESCA medium supplemented with Myricetin-derivatives at the indicated concentrations at 30 °C for 7 days. As a control, 1% DMSO without any compounds was supplemented in the medium. A photograph of the 0.2% CV-stained biofilms is shown. (b) Biofilm biomass was quantified by measuring absorbance at 595 nm. (c) Growth of E. coli BW25113 was monitored in the presence of EGCG at the indicated concentrations. As a control, 1% DMSO was added to the culture. (b,c) The means and standard deviations from at least triplicate determinations are represented. (d) Basic chemical structure of the compounds used in this study and summary of structure-function relationship among them are shown. IC₅₀ was calculated as previously described²⁶. DB, double bond; Rham, rhamnose; Gal, gallate.