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. 2018 May 31;8:8452. doi: 10.1038/s41598-018-26748-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of EGCG on biofilm formation by a clinically isolated strain of E. coli. (a) Biofilms of E. coli O157:H7 Sakai were formed in the presence of the indicated concentrations of EGCG and quantified as described in Fig. 2. (b) Effect of EGCG on growth of the strain was investigated at 30 °C in YESCA medium supplemented with the indicated concentrations of EGCG. As a control, 1% DMSO without any compounds was supplemented in the medium. Absorbance at 600 nm every 30 min was measured. The means and standard deviations from at least triplicate determinations are represented.