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. 2018 May 25;9:1105. doi: 10.3389/fmicb.2018.01105

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Copyright © 2018 Song, Zhang, Cai, Lv, Zhao, Wei and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction of a ΔhtsA mutant and ΔhtsA-htsA revertant. (A) The arrangement of the shr, shp, and htsABC genes in the operon. (B) PCR analysis of fragments amplified from chromosomes of GAS strains. The PCR fragment detected in the ΔhtsA mutant was 303-bp smaller than that in the wild type, and the PCR fragment detected in the ΔhtsA-htsA revertant was identical to that in wild type. (C) Western blotting analysis of GAS strains. HtsA protein was expressed in wild type and ΔhtsA-htsA strains but not in the ΔhtsA mutant. Shp was used as a loading control.