Fig. 4. Subcellular localization of SlAN11 protein.

. The PCR-amplified GFP fragment, encoding green fluorescent protein, was cloned into XbaI and SalI sites of pTEX to generate pTEX-GFP. The coding sequences of SlAN11 genes were PCR-amplified from tomato leaf cDNA and cloned in the pTEX-GFP vector to generate SlAN11-GFP construct. Subcellular localization assay was carried out via transformation of tomato protoplasts. After DAPI staining the protoplasts was examined using confocal microscope to simultaneously capture DAPI and GFP signals. pTEX-GFP construct was included as control. Left to right: green, GFP fluorescence; red, chlorophyll autofluorescence; blue, nucleus stained with DAPI; merged, combined fluorescence from GFP, chlorophyll, and DAPI. Bars, 5 μm