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. 2018 Jun 1;5:27. doi: 10.1038/s41438-018-0032-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Phenotypes of proanthocyanidin accumulation in seeds from plants of wild-type (WT), 35S::SlAN11 (SlAN11-OX), and SlAN11-RNAi (SlAN11-Ri) transgenic lines. b Germination phenotype of wild-type (WT), 35S::SlAN11 (OX), and SlAN11-RNAi (Ri) transgenic seeds in the different concentrations of ABA. c Quantitative analysis of anthocyanin contents of seeds from wild-type, 35S::SlAN11, and SlAN11-RNAi transgenic lines. Results represent mean values ± SD from three biological replicates. Asterisks indicate statistically significant differences (**P < 0.01, ***P < 0.001; t-test). d Germination ratio of WT and transgenic T₂ seeds at the different concentrations of ABA. The percentage of seeds showing root emergence was scored 8 days post stratification. Standard error bars represent three independent experiments. e Germination ratio of WT and transgenic T₂ seeds in 1/2 MS plates with 2.5 μM ABA. Germinated seeds (radicle protruding) were counted daily for 12 days. Germination ratio refers to the number of germinated seeds as a proportion of the total number of seeds. Standard deviation bars represent three independent experiments