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. 2018 May 31;8:8499. doi: 10.1038/s41598-018-26749-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the internalisation of phage clone into A431 and NHDF cells with immunofluorescence microscopy. The cell lines used are labelled on the left. A431 and NHDF cells without phage served as negative controls. Purified phage (1 × 10¹² pfu) carrying the peptide NRPDSAQFWLHH was added to the cells. After incubation at 37 °C for 72 h, the phage was detected with the mouse anti-M13 monoclonal antibody, followed by the goat anti-mouse IgG conjugated to Alexa -Fluor^® 488.