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. 2018 May 31;8:8499. doi: 10.1038/s41598-018-26749-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Delivery of tHBcAg VLNPs into A431 cells with peptide NRPDSAQFWLHHGGGSLLGRMKGA containing the nanoglue. (a) SDS-PAGE of tHBcAg conjugated to peptide NRPDSAQFWLHHGGGSLLGR-MKGA. Lanes M: molecular mass markers (kDa), A1: tHBcAg, A2: tHBcAg plus peptide NRPDSAQFWLH-HGGGSLLGRMKGA without cross-linker, A3: tHBcAg plus cross-linker without peptide, A4: tHBcAg plus cross-linker and peptide NRPDSAQFWLHHGGGSLLGRMKGA. The arrow shows the tHBcAg monomer conjugated to the peptide. The full length gel is shown in Supplementary Figure S1. (b) Delivery of tHBcAg VLNPs conjugated to peptide NRPDSAQFWLHHGGGSLLGRMKGA into A431 cells. tHBcAg was detected with the mouse anti-HBcAg monoclonal antibody, followed by the FITC-conjugated goat anti-mouse antibody. A431 cells incubated with tHBcAg VLNPs that had been conjugated to the peptide showed strong green fluorescent dots. The samples added to the cells are labelled on the left of figure.