Figure 5.
Heart structure of P. clarkii following 96 h exposure to a control solution (A) and 0.24 mg/L pymetrozine (B,C). Control hearts with normal structures of myocardium (m), adventitia (a) and multinucleated and branched myocardial fibers (mf). Note the netlike adventitia with cytoplasm-lacking and eccentrically located nuclei of the epithelial cells. ((A), 200×). ec, epithelial cell. Damaged hearts showing hemocytic infiltration (hi), degeneration of the myocardial fibers (dm) ((B), 200×), epithelial hyperplasia of the adventitia (ah) ((C), 200×), and vacuolation (v) (B,C). m, myocardium; n, nuclei; H&E stain, scale bars = 100 μm.