Skip to main content
. Author manuscript; available in PMC: 2019 May 9.
Published in final edited form as: Cell Host Microbe. 2018 May 9;23(5):607–617.e6. doi: 10.1016/j.chom.2018.04.007

Figure 3. The EHEC 86–24 lambdoid phage Cro activates the LEE.

Figure 3

(A) qRT-PCR gene of LEE genes (ler, tir, sepL and espA) from RNA extracted from EHEC (WT, Δcro, and complemented strain) grown under anaerobic conditions in DMEM low glucose. (n=6, error bars, standard deviation, P<0.01).

(B) Western blot for of the LEE-encoded and T3SS secreted protein EspA from secreted proteins and whole cell lysates of EHEC (WT, Δcro, ΔespA, and Δcro complemented [Δcro-pcro]) grown under anaerobic conditions in DMEM low glucose. Bovine serum albumin (BSA) was used as loading control for secreted proteins, and αRpoA was the loading control for whole cell lysates.

(C) FAS assay showing AE lesions in EHEC WT, Δcro and complemented mutant on infected HeLa cells.

(D) Quantification of FAS Log transformation of pedestal/count numbers in EHEC WT, mutants and complemented strains on infected HeLa cells (n=150, Error bars, standard deviation, P<0.01). Subjects with asterisks (**) indicates statistical significance at P<0.01 (Error bars, standard deviation, Student’s t-test). See also Figure S3.