(A) Cartoon depicting the strategy utilized to exchange the EHEC ler promoter region to C. rodentium ler promoter region. The promoter was successfully exchanged using the λ recombination method, obtaining C.r-j11 and C.r-j11-pcro strains.
(B) Weight loss or gain from baseline (weight at day 0) over the course of infection (Blue: mock infected, Red: C. rodentium DBS100 wt, Purple: C. rodentium C.r-j11 pWSK129, and Green: C. rodentium C.r-j11-pcro) (n=10, error bars, standard deviation).
(C) Day 5 weight loss or gain statistical analysis comparing DBS100 against C.r-j11 pWSK129 and C.r-j11-pcro showing no statistical difference (NS).
(D) Day 6 weight loss or gain statistical analysis, comparing DBS100 against C.r-j11 pWSK129, and C.r-j11-pcro were depicting statistical difference at P<0.05.
(E) Day 7 weight loss or gain statistical analysis comparing DBS100 against C.r-j11pWSK129 and C.r-j11-pcro. The analysis shows statistical difference between DBS100 and C.r-j11-pcro (P<0.01), and no significance were observed comparing DBS100 and C.r-j11 pWSK129.
The nonparametric Mann-Whitney U test was used to determine the statistical significance using Graphpad prism software. See also Figure S6.