Table 3.

Clotting activity relative to no PS of FIXa at Varied PS Concentrations in FIX-PS Double Immunodepleted Plasma

FIXa Construct	0 nmole/liter PS	150 nmole/liter PS	300 nmole/liter PS
WT FIXa	1 ± 0.074	0.288 ± 0.006	0.121 ± 0.002
R150A	1 ± 0.008	0.310 ± 0.005	0.098 ± 0.004
K132A R170A	1 ± 0.065	0.677 ± 0.025	0.417 ± 0.019

The table shows relative FIXa coagulant activity with respect to no PS binding obtained from titration of FIX-PS double immunodepleted plasma with 100 pmole/liter FIXaWT, R150A, and K132AR170A in the absence and presence of PS. The values represent the relative coagulant activity of 100 pmole/liter FIXa WT, in the absence of protein S compared with activity after supplementation of 150 or 300 nM PS into the FIX-PS double depleted plasma. Results are reported as mean relative coagulation activity fraction ± STDEV (n = 3 replicates for each).