Table 1. Bacterial strains and plasmids used in this study.
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Bacteria | ||
| S. aureus JE2 | Most common CA-MRSA isolate, resistant to methicillin/oxacillin | [57] |
| lpdA | JE2 with mutation in the lpdA (Kanr) | This study |
| lpdA+pCU-bkd | JE2 : lpdA complemented with pCU-bkd plasmid (Kanr, Camr) | This study |
| NE1724 | JE2 with a transposon insertion in the pdhA gene (Ermr) | [35] |
| pdhA+pCU-pdh | JE2 : pdhA complemented with pCU-pdh plasmid (Ermr, Camr) | This study |
| lpdA : pdhA | JE2 with mutation in the lpdA and pdhA genes (Kanr, Ermr) | This study |
| lpdA-pdhA+pCU-bkd | JE2Δ(pdhA+lpdA)complemented with pCU-bkd (Kanr, Ermr, Camr) | This study |
| lpdA-pdhA+pCU-pdh | JE2Δ(pdhA+lpdA)complemented with pCU-pdh (Kanr, Ermr, Camr) | This study |
| Plasmids | ||
| pGEM-T | An E. coli cloning plasmid | Promega |
| pCU1 | A shuttle vector (Ampr in E. coli, Camr in S. aureus) | [36] |
| pCU-bkd | Plasmid pCU1 containing a 5.1-kb DNA fragment containing all four genes of the S. aureus bkd locus | [23] |
| pCU-pdh | Plasmid pCU1 containing a 5.9-kb DNA fragment containing all four genes of the S. aureus pdh locus | This study |
Kanr, kanamycin resistant; Ermr, erythromycin resistant; Camr, chloramphenicol resistant.