Table 2. Fatty acid profiles of wild-type JE2 and isogenic lpdA, pdhA and lpdA-pdhA mutants and complemented strains.
|
Fatty acid |
% (wt/wt) of total fatty acids | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Wild type | lpdA | lpdA with | pdhA | pdhA with | lpdA : pdhA | ΔlpdA : pdhA with | ||||
| pCU-bkd | pCU-pdh | pCU-bkd | pCU-pdh | pCU-bkd | pCU-pdh | |||||
| Ante-iso C15 : 0 | 28.16 | 11.56 | 26.72 | 24.19 | 37.09 | 37.41 | 29.23 | 10.84 | 34.50 | 23.29 |
| Ante-iso C17 : 0 | 2.70 | nd | 2.88 | 2.99 | 10.00 | 11.16 | 2.86 | nd | 7.88 | 1.35 |
| Ante-iso C19 : 0 | nd | nd | nd | nd | 2.30 | 2.69 | nd | 3.10 | 1.88 | nd |
| Ante-iso | 30.86 | 11.56 | 29.60 | 27.18 | 49.39 | 51.26 | 32.09 | 13.94 | 44.26 | 24.64 |
| Straight even | 45.34 | 63.98 | 49.80 | 47.50 | 19.10 | 20.55 | 47.83 | 58.87 | 22.51 | 56.52 |
| Iso odd | 14.76 | 3.08 | 6.91 | 5.72 | 26.26 | 24.11 | 12.11 | nd | 17.50 | 11.25 |
| Iso even | 4.47 | 16.15 | 13.69 | 19.60 | 4.00 | 2.64 | 5.28 | 23.61 | 13.99 | 5.27 |
| Iso | 19.23 | 19.23 | 20.60 | 25.32 | 30.26 | 26.75 | 17.39 | 23.61 | 31.49 | 16.52 |
| Ante-iso : iso ratio | 1.60 | 0.60 | 1.44 | 1.07 | 1.63 | 1.92 | 1.85 | 0.59 | 1.41 | 1.49 |
| BCFA | 50.09 | 30.79 | 50.20 | 52.50 | 79.65 | 78.01 | 49.48 | 37.55 | 75.75 | 41.16 |
| SCFA | 46.89 | 63.98 | 49.80 | 47.50 | 19.10 | 20.55 | 49.08 | 58.87 | 22.51 | 57.55 |
nd, none detected.
Values indicate the average of two independent experiments.