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An example of the sensorgram of immobilization of a bait protein (cytochrome b5). 1 RU corresponds to 1 pg of the bound substance per 1 mm2 of the chip surface. The following parameters of the immobilization protocol can be optimized by using the SPR biosensor: (a) a pH value of the immobilization buffer; (b) concentrations of the bait protein (bold dashed line shows different concentrations of the bait protein); (c) time of injection.
