Table 2.
Quantitative interaction of purified proteins with isatin analogues immobilized on the Biacore optical biosensor chip (modified from [16]).
| Protein | 5-aminoisatin Kd, M |
5-aminocaproylisatin Kd, M |
|---|---|---|
| Rabbit GAPDH intact | (2.2 ± 0.6) × 10−6 | - |
| Rabbit GAPDH oxidized | (8.9 ± 1.9) × 10−6 | - |
| Human recombinant cytokeratin 14 | ND | (7 ± 5) × 10−7 |
| Human recombinant cytokeratin 8 | (6.1 ± 0.3) × 10−7 | ND |
| Human recombinant peroxiredoxin | (8.8 ± 0.4) × 10−8 | (5.3 ± 0.2) × 10−7 |
| Rabbit creatine phosphokinase | (2.9 ± 0.4) × 10−7 | (7.7 ± 3.1) × 10−8 |
| Rabbit glycogen phosphorylase | (3.0 ± 0.6) × 10−5 | (4.6 ± 3.8) × 10−5 |